General Information

  • ID:  hor005948
  • Uniprot ID:  P12797
  • Protein name:  Megascoliakinin
  • Gene name:  NA
  • Organism:  Megascolia flavifrons (Garden dagger wasp) (Solitary wasp)
  • Family:  Bradykinin-related peptide family
  • Source:  Animal
  • Expression:  Expressed by the venom gland.
  • Disease:  NA
  • Comments:  NA
  • Taxonomy:  Megascolia (genus), Scoliidae (family), Scolioidea (superfamily), Aculeata (infraorder), Apocrita (suborder), Hymenoptera (order), Endopterygota (cohort), Neoptera (infraclass), Pterygota (subclass), Dicondylia, Insecta (class), Hexapoda (subphylum), Pancrustacea, Mandibulata, Arthropoda (phylum), Panarthropoda, Ecdysozoa, Protostomia, Bilateria, Eumetazoa, Metazoa (kingdom), Opisthokonta, Eukaryota (superkingdom), cellular organisms
  • GO MF:  GO:0003674 molecular_function; GO:0090729 toxin activity
  • GO BP:  GO:0035821 modulation of process of another organism; GO:0045776 negative regulation of blood pressure; GO:0045987 positive regulation of smooth muscle contraction
  • GO CC:  GO:0005576 extracellular region; GO:0005615 extracellular space

Sequence Information

  • Sequence:  RPPGFTPFRKA
  • Length:  11(1-11)
  • Propeptide:  RPPGFTPFRKA
  • Signal peptide:  NA
  • Modification:  NA
  • Glycosylation:  NA
  • Mutagenesis:  NA

Activity

  • Function:  [Thr6-bradykinin]: Inhibits ACE with a Ki of 1.6 uM, and targets B2 bradykinin receptor (BDKRB2). Provokes contraction of smooth muscle preparation (ileum). In vivo, induces an early hyperalgesic effects in living rats after intraplantar injection (By sim
  • Mechanism:  NA
  • Cross BBB:  NA
  • Target:  NA
  • Target Unid:  NA
  • IC50: NA
  • EC50: NA
  • ED50: NA
  • kd: NA
  • Half life: NA

Structure

  • Disulfide bond:  NA
  • Structure ID:  NA

  • Structure: (PDB_ID-from https://www.rcsb.org/; AlphaFold_DB_ID-from https://alphafold.ebi.ac.uk/; hordbxxxxxx_AF2.pdb was predicted structure by AlphaFold2; hordbxxxxxx_ESM.pdb was predicted structure by ESMFold)
    •    hor005948_AF2.pdbhor005948_ESM.pdb

Physical Information

Mass: 145203 Formula: C60H92N18O13
Absent amino acids: CDEHILMNQSVWY Common amino acids: P
pI: 12.52 Basic residues: 3
Polar residues: 2 Hydrophobic residues: 3
Hydrophobicity: -103.64 Boman Index: -2925
Half-Life: 1 hour Half-Life Yeast: 2 min
Half-Life E.Coli: 2 min Aliphatic Index 9.09
Instability Index: 6161.82 Extinction Coefficient cystines: 0
Absorbance 280nm: 0

Literature

  • PubMed ID:  3617088
  • Title:  Two kinins isolated from an extract of the venom reservoirs of the solitary wasp Megascolia flavifrons.
  • PubMed ID:  27096870
  • Title:  Peptide Toxins in Solitary Wasp Venoms.